REPOPULATION OF POSTMITOTIC NUCLEOLI BY PREFORMED RNA
نویسندگان
چکیده
منابع مشابه
Repopulation of Postmitotic Nucleoli by Preformed Rna
The reconstruction of the nucleolus after mitosis was analyzed by electron microscopy in cultured mammalian (L929) cells in which nucleolar RNA synthesis was inhibited for a 3 h period either after or before mitosis. When synchronized mitotic cells were plated into a concentration of actinomycin D sufficient to block nucleolar RNA synthesis preferentially, nucleoli were formed at telophase as u...
متن کاملREPOPULATION OF POSTMITOTIC NUCLEOLI BY PREFORMED RNA II . Ultrastructure
The reconstruction of the nucleolus after mitosis was analyzed by electron microscopy in cultured mammalian (L929) cells in which nucleolar RNA synthesis was inhibited for a 3 h period either after or before mitosis . When synchronized mitotic cells were plated into a concentration of actinomycin D sufficient to block nucleolar RNA synthesis preferentially, nucleoli were formed at telophase as ...
متن کاملRepopulation of the Postmitotic Nucleolus by Preformed Rna
This study is concerned with the fate of the nucleolar contents, particularly nucleolar RNA, during mitosis Mitotic cells harvested from monolayer cultures of Chinese hamster embryonal cells, KB6 (human) cells, or L929 (mouse) cells were allowed to proceed into interphase in the presence or absence (control) of 0.04-0 08 microg/ml of actinomycin D, a concentration which preferentially inhibits ...
متن کاملPostmitotic reconstruction of nucleoli in culture cells with UV-microbeam photoinactivated centrosome.
Ultraviolet microirradiation of one of the poles of the mitotic spindle of PK cells was performed 1 min after the onset of the anaphase. Formation of the nucleolus in the telophase and G1 period was studied by vital observation, electron microscopy and indirect immunofluorescence using antibodies against B23 protein. Sister cells with nonirradiated centrosomes and cells with partially irradiate...
متن کاملThe DNA topoisomerase I inhibitor camptothecin blocks postmitotic reformation of nucleoli in mammalian cells.
During mitotic prophase of most eukaryotic cells, transcription of the rRNA genes ceases and nucleolar structure breaks down. While some nucleolar protein components remain associated with the chromosomal NORs, others bind to the surface of the chromosomes or become dispersed throughout the cytoplasm of dividing cells (see Tab. I in [1]). During telophase, nucleoli reform progressively at the N...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1973
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.58.1.54